cd79a pe  (Novus Biologicals)

Journal: Scientific Reports

Article Title: Single-cell RNA sequencing reveals that a high salt diet increases IL6R-JAK1-STAT3 gene signaling pathway activity in intestinal B cells and Tfh cells of salt-sensitive hypertension animal model

doi: 10.1038/s41598-025-11100-z

Figure Lengend Snippet: HSD intake increases B cell population in the SILPs of SS rats. ( A , C ) UMAP plots display clusters of CD45+ cells in the SILPs. ( A ) Annotations indicate cell types based on gene expression. ( B ) Dot plot shows representative gene expression for each cell type. ( C ) Red dots represent the NSD group, and blue dots represent the HSD group. ( D ) Bar plot shows the proportion of immune cells in the SILPs ( n = 4/group). # p = 0.05 by t-test. ( E ) Bar plots represent the distribution of B cells (CD45+ CD79a+) in the SILPs ( n = 4/group) using flow cytometry. * p < 0.05 in a t-test.

Article Snippet: Antibodies used for staining B cells and Tfh cells in this study as follows : CD45-PE/Cyanine7 (Biolgend, San Diego, CA, USA, 202214), CD79a-PE (Novus Biologicals, Centennial, CO, USA, NBP2-54469PE), CD3-BV421 (BD, Franklin Lakes, NJ, USA, 563948), CD4-APC Cy7 (BD, 565432), CXCR5-unconjugated (Invitrogen, Waltham, Massachusetts, USA, MA5-41258), and secondary antibody-FITC (Invitrogen, A-11034).

Techniques: Gene Expression, Flow Cytometry

Journal: BMC Veterinary Research

Article Title: Diagnosis of canine B-cell chronic lymphoid leukemia with a CD21 negative phenotype using the LT21 clone CD21 antibody in flow cytometry: a case report

doi: 10.1186/s12917-024-04335-x

Figure Lengend Snippet: Antibodies used for flow cytometry

Article Snippet: CD79a monoclonal antibody-PE (Mouse IgG1, κ) , HM47 , Invitrogen , 12-0792-42.

Techniques: Staining, Control, Negative Control

Journal: BMC Veterinary Research

Article Title: Diagnosis of canine B-cell chronic lymphoid leukemia with a CD21 negative phenotype using the LT21 clone CD21 antibody in flow cytometry: a case report

doi: 10.1186/s12917-024-04335-x

Figure Lengend Snippet: Amounts of antibodies used for flow cytometry analysis

Article Snippet: CD79a monoclonal antibody-PE (Mouse IgG1, κ) , HM47 , Invitrogen , 12-0792-42.

Techniques: Flow Cytometry, Staining, Control, Negative Control

Journal: BMC Veterinary Research

Article Title: Diagnosis of canine B-cell chronic lymphoid leukemia with a CD21 negative phenotype using the LT21 clone CD21 antibody in flow cytometry: a case report

doi: 10.1186/s12917-024-04335-x

Figure Lengend Snippet: Immunophenotyping of peripheral mononuclear cells before chemotherapy. Peripheral blood mononuclear cells isolated from the canine patient were immunophenotyped by flow cytometric analysis using FITC-conjugated mouse anti-dog CD3/PE-conjugated mouse anti-CD79A, FITC-conjugated rat anti-dog CD4/PE-conjugated rat anti-dog CD8 monoclonal antibody, FITC-conjugated mouse anti-dog CD21/PE-conjugated mouse anti-CD79A, PE-conjugated mouse anti-canine CD34, FITC-conjugated rat ant-canine CD45, FITC-conjugated rat anti-canine MHC class II, and FITC-conjugated mouse anti-human CD14 antibodies. Nonspecific binding of targeted antibodies to cell surface antigens was estimated using isotype control antibodies. Gating was performed with reference to populations of unstained, single stained, and isotype controls. FITC: fluorescein isothiocyanate; PE: phycoerythrin

Article Snippet: CD79a monoclonal antibody-PE (Mouse IgG1, κ) , HM47 , Invitrogen , 12-0792-42.

Techniques: Isolation, Binding Assay, Control, Staining

Journal: iScience

Article Title: Comparative transcriptomic analysis of bovine mesenchymal stromal cells reveals tissue-source and species-specific differences

doi: 10.1016/j.isci.2024.108886

Figure Lengend Snippet:

Article Snippet: Mouse Anti-CD79a conj. PE , Bio-Rad , Cat# MCA2538PE; RRID: AB_931716.

Techniques: Control, Recombinant, Saline, Staining, cDNA Synthesis, SYBR Green Assay, RNA Sequencing, Software, Light Microscopy, Microscopy, Flow Cytometry, Real-time Polymerase Chain Reaction